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KMID : 0359320100500030221
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Korean Journal of Veterinary Research
2010 Volume.50 No. 3 p.221 ~ p.229
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Polymorphisms of the poly(C)-tract of porcine encephalomyocarditis virus (EMCV) isolated in Korea
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Hyun Bang-Hun
Kim Hyo-Jin
Kim In-Joong
Pyo Hyun-Mi
Kim Sun-Mi
Kim Seong-Hee
Kim Jae-Jo
Lim Seong-In
Song Jae-Young
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Abstract
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Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C’s) with sequences consisting of C9, C10, C13, C14, C16, C20, CUC11, C8UCUC3UC10, C9UCUC3UC10, C10UCUC3UC10, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.
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KEYWORD
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encephalomyocarditis virus, Korean isolate, poly(C) tract, polymorphism
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